ECA, the enterobacterial common antigen

Enterobacterial common antigen.

INTRODUCTION .............. ................................ 591 DISCOVERY AND DEFINITIONS ............................................. 591 SEROLOGICAL METHODS ............................................. 592 Hemagglutination (HA), Hemolysis, and Hemagglutination Inhibition (HAI) ... 592 Other Serological Methods ............................................. 593 CHEMISTRY .......................

Isolation and chemical characterization of the enterobacterial common antigen.

The isolation of the soluble form of the Enterobacteriaceae common antigen from Salmonella montevideo was achieved by a combination of the phenol/water method and the phenol/chloroform/ petroleum ether extraction procedure. A phenol-soluble fraction highly enriched in the antigen was obtained which was further purified by DEAE-cellulose chromatography. A fraction eluted with 0.9 M ammonium acet...

Biosynthesis of Enterobacterial Common Antigen in Esc~erichia coli

Twelve independent TnlO insertion mutants of Escherichia coli K12 were isolated that were defective in the synthesis of enterobacterial common antigen (ECA). The mutants were identified by screening a random pool of TnlO insertion mutants for their ECA phenotype using a colony-immunoblot assay. All 12 of the TnlO insertion mutants were found to be located in the chromosomal region of the rff-rf...

Serological and immunological properties of isolated enterobacterial common antigen.

Enterobacteriaceae common antigen (sometimes abbreviated to ECA) isolated from Salmonella montevideo by a combination of phenol/water and phenol/chloroform/petroleum ether extraction procedures and further purified by ion-exchange chromatography was investigated relative to its serological and immunological properties. The isolated material was highly active in coating erythrocytes for agglutin...

O-ACETYLATION OF THE ENTEROBACTERIAL COMMON ANTIGEN (ECA) POLYSACCHARIDE IS CATALYZED BY THE PRODUCT OF THE yiaH GENE OF Escherichia coli K-12